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Regeneration was advanced in the CDAHFD-treated PHx mouse model (A) Body weight changes in the CD subgroups. (B–C) Liver weight and liver index changes in the CD subgroups. (D–G) Relative mRNA levels of Cyclin D1 , Cyclin B1 , Cyclin E1 , and Ki 67 in the CD subgroups. (H) Hepatic protein levels of pathways involved in regeneration. (I–K) Gray value levels of hepatic proliferative proteins. Data were expressed as mean ± SD. One-way ANOVA between multiple groups ( n = 5, ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001, compared with CD-D0 subgroup).

Journal: iScience

Article Title: Endogenous phosphatidylcholine 16:0/20:4 increases to promote liver regeneration in hepatectomy

doi: 10.1016/j.isci.2026.116962

Figure Lengend Snippet: Regeneration was advanced in the CDAHFD-treated PHx mouse model (A) Body weight changes in the CD subgroups. (B–C) Liver weight and liver index changes in the CD subgroups. (D–G) Relative mRNA levels of Cyclin D1 , Cyclin B1 , Cyclin E1 , and Ki 67 in the CD subgroups. (H) Hepatic protein levels of pathways involved in regeneration. (I–K) Gray value levels of hepatic proliferative proteins. Data were expressed as mean ± SD. One-way ANOVA between multiple groups ( n = 5, ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001, compared with CD-D0 subgroup).

Article Snippet: Cyclin D1 Rabbit mAb , ABclonal , Cat# A19038, RRID: AB_2862530.

Techniques:

PC metabolism correlated well with the regeneration peak in the CDAHFD-treated PHx mouse model (A–B) The mRNA levels of Cyclin D1 and Cyclin E1 . (C–F) The mRNA levels of Pcyt1a , Pemt , Chka , and Cept1 . (G) Spearman’s correlation analysis between proliferation phenotype and PC synthesis in the CDAHFD-treated PHx mice. Data were expressed as mean ± SD. One-way ANOVA between multiple groups. n = 5, ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001, compared with CDAHFD-D0 subgroup.

Journal: iScience

Article Title: Endogenous phosphatidylcholine 16:0/20:4 increases to promote liver regeneration in hepatectomy

doi: 10.1016/j.isci.2026.116962

Figure Lengend Snippet: PC metabolism correlated well with the regeneration peak in the CDAHFD-treated PHx mouse model (A–B) The mRNA levels of Cyclin D1 and Cyclin E1 . (C–F) The mRNA levels of Pcyt1a , Pemt , Chka , and Cept1 . (G) Spearman’s correlation analysis between proliferation phenotype and PC synthesis in the CDAHFD-treated PHx mice. Data were expressed as mean ± SD. One-way ANOVA between multiple groups. n = 5, ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001, compared with CDAHFD-D0 subgroup.

Article Snippet: Cyclin D1 Rabbit mAb , ABclonal , Cat# A19038, RRID: AB_2862530.

Techniques:

PC (16:0/20:4) facilitated the proliferation of Hepa 1-6 and the mouse model (A–B) CCK8 determination of Hepa 1-6 cells after 24 and 48 h of PC (16:0/20:4) challenge. (C–D) Number of Hepa 1-6 cells after 24 and 48 h of PC (16:0/20:4) challenge. (E) H&E staining of Hepa 1-6 cells after 24 and 48 h of PC (16:0/20:4) challenge. Scale bars, 50 μm. (F–I) The mRNA levels of Cyclin D1 , Ki 67 , Cyclin B1 , and Cyclin E1 . (J–L) The mRNA levels of Cyclin D1 , E1 , and Pcna determined using qPCR in the mouse liver challenged with PC (16:0/20:4). (M) Hepatic protein levels of CYCLIN D1 and PCNA in the mouse liver challenged with PC (16:0/20:4). Data were expressed as mean ± SD. One-way ANOVA between multiple groups. n = 5, ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001, compared with 0 μM group.

Journal: iScience

Article Title: Endogenous phosphatidylcholine 16:0/20:4 increases to promote liver regeneration in hepatectomy

doi: 10.1016/j.isci.2026.116962

Figure Lengend Snippet: PC (16:0/20:4) facilitated the proliferation of Hepa 1-6 and the mouse model (A–B) CCK8 determination of Hepa 1-6 cells after 24 and 48 h of PC (16:0/20:4) challenge. (C–D) Number of Hepa 1-6 cells after 24 and 48 h of PC (16:0/20:4) challenge. (E) H&E staining of Hepa 1-6 cells after 24 and 48 h of PC (16:0/20:4) challenge. Scale bars, 50 μm. (F–I) The mRNA levels of Cyclin D1 , Ki 67 , Cyclin B1 , and Cyclin E1 . (J–L) The mRNA levels of Cyclin D1 , E1 , and Pcna determined using qPCR in the mouse liver challenged with PC (16:0/20:4). (M) Hepatic protein levels of CYCLIN D1 and PCNA in the mouse liver challenged with PC (16:0/20:4). Data were expressed as mean ± SD. One-way ANOVA between multiple groups. n = 5, ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001, compared with 0 μM group.

Article Snippet: Cyclin D1 Rabbit mAb , ABclonal , Cat# A19038, RRID: AB_2862530.

Techniques: Staining

PC (16:0/20:4) facilitated the proliferation of Huh-7 cells (A–B) CCK8 determination of Huh-7 cells after 24 and 48 h of PC (16:0/20:4) challenge. (C–D) The number of Huh-7 cells after 24 and 48 h of PC (16:0/20:4) challenge. (E) H&E staining of Huh-7 cells after 24 and 48 h of PC (16:0/20:4) challenge. Scale bars, 50 μm. (F–I) The relative mRNA levels of Cyclin D1 , Ki 67 , Cyclin B1 , and Cyclin E1 . Data were expressed as mean ± SD. One-way ANOVA between multiple groups. n = 5, ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001, compared with 0 μM group.

Journal: iScience

Article Title: Endogenous phosphatidylcholine 16:0/20:4 increases to promote liver regeneration in hepatectomy

doi: 10.1016/j.isci.2026.116962

Figure Lengend Snippet: PC (16:0/20:4) facilitated the proliferation of Huh-7 cells (A–B) CCK8 determination of Huh-7 cells after 24 and 48 h of PC (16:0/20:4) challenge. (C–D) The number of Huh-7 cells after 24 and 48 h of PC (16:0/20:4) challenge. (E) H&E staining of Huh-7 cells after 24 and 48 h of PC (16:0/20:4) challenge. Scale bars, 50 μm. (F–I) The relative mRNA levels of Cyclin D1 , Ki 67 , Cyclin B1 , and Cyclin E1 . Data were expressed as mean ± SD. One-way ANOVA between multiple groups. n = 5, ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001, compared with 0 μM group.

Article Snippet: Cyclin D1 Rabbit mAb , ABclonal , Cat# A19038, RRID: AB_2862530.

Techniques: Staining

PC (16:0/20:4) facilitated the proliferation of human organoids and the clinical evidence (A) Representative images of growing human liver organoids on day 1 and day 4 after PC (16:0/20:4) challenge. Scale bars, 250 μm. (B) Proliferation of growing human liver organoids determined by CellTiter-Glo3D ( n = 3/group). (C–E) The mRNA levels of Cyclin D1 , Ki 67 , and Cyclin B1 after 3 days of PC (16:0/20:4) challenge ( n = 3/group). (F) Concentration of the serum PC (16:0/20:4) among patients with MASLD in comparison with that in healthy volunteers ( n = 30–32/group). (G) Concentration of the serum PC (16:0/20:4) increased in PHx patients whose samples on day 1 were not missing. (H) Concentration of the serum PC (16:0/20:4) increased in PHx patients whose samples on day 1 were missing. Data were expressed as mean ± SD. One-way ANOVA between multiple groups. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001, compared with 0 μM group.

Journal: iScience

Article Title: Endogenous phosphatidylcholine 16:0/20:4 increases to promote liver regeneration in hepatectomy

doi: 10.1016/j.isci.2026.116962

Figure Lengend Snippet: PC (16:0/20:4) facilitated the proliferation of human organoids and the clinical evidence (A) Representative images of growing human liver organoids on day 1 and day 4 after PC (16:0/20:4) challenge. Scale bars, 250 μm. (B) Proliferation of growing human liver organoids determined by CellTiter-Glo3D ( n = 3/group). (C–E) The mRNA levels of Cyclin D1 , Ki 67 , and Cyclin B1 after 3 days of PC (16:0/20:4) challenge ( n = 3/group). (F) Concentration of the serum PC (16:0/20:4) among patients with MASLD in comparison with that in healthy volunteers ( n = 30–32/group). (G) Concentration of the serum PC (16:0/20:4) increased in PHx patients whose samples on day 1 were not missing. (H) Concentration of the serum PC (16:0/20:4) increased in PHx patients whose samples on day 1 were missing. Data were expressed as mean ± SD. One-way ANOVA between multiple groups. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001, compared with 0 μM group.

Article Snippet: Cyclin D1 Rabbit mAb , ABclonal , Cat# A19038, RRID: AB_2862530.

Techniques: Concentration Assay, Comparison